Wheat germ agglutinin (WGA), Fluorescein labeled, succinylated

Succinylated Wheat Germ Agglutinin (WGA), Fluorescein, 5 mg, is a conjugated form of WGA that has been reported to have properties distinct from the native lectin. Evidence suggests it does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures. Fluorescein-labeled Succinylated Wheat Germ Agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin and is supplied essentially free of unconjugated fluorochromes. Fluorescein has an excitation maximum of 495 nm and an emission maximum of 515 nm.