Wisteria floribunda lectin (WFA, WFL), Agarose bound

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound, 2 ml, is an agarose-conjugated form of WFL. It is supplied as a 1:1 suspension in buffer and comprises a matrix of heat-stable, crosslinked 4% agarose beads that is stable in solutions at pH 3–11 as well as many organic solvents. Covalent attachment of WFL to the agarose beads (using a hydrophilic spacer arm) preserves lectin activity and minimizes conformational changes that might result in non-specific or hydrophobic interactions. This also ensures that conjugated proteins are not leached off the beads by Tris or other routinely used buffers. Because no residual charge is present after conjugation, non-specific binding to the matrix is minimized.