Ion exchange (IEX) chromatography is a powerful technique that separates molecules based on their surface charge. The stationary phase contains charged functional groups that interact electrostatically with oppositely charged analytes.
In cation exchange chromatography (CEX), positively charged molecules bind to a negatively charged stationary phase.
In anion exchange chromatography (AEX), negatively charged molecules bind to a positively charged stationary phase.
IEX is widely applied to the purification of proteins, peptides, oligonucleotides, and charged small molecules. By fine-tuning parameters such as pH, buffer composition, and salt gradient, researchers can precisely control selectivity, resolution, and elution profiles for optimal separation performance.
YMC’s BioPro IEX and BioLC column families deliver reliable, high-resolution separations from analytical to preparative scale.
Column Types:
YMC-BioPro IEX columns are available with porous and non-porous particles for strong anion exchange (SAX) and strong cation exchange (SCX).
Columns packed with the porous materials (QA & SP) are advantageous for high-load analytical separations and laboratory-scale purification as they maintain strong peak shape even with increased load.
The non-porous formats (QF & SF) are highly efficient, and are therefore ideal for high-throughput analytical separations requiring high resolution.
Specifications:
- Charged Group: -CH2N+(CH3)3
- Porosity: Non-porous
- pH Range: 2-12
- Particle Size: 5 µm
- Column Format: PEEK Hardware (Analytical)
