Cannabis and CBD-related Application Notes

 

This section is organized by analysis type, select from:



Potency

Add Vitamin E Acetate Screening to Your Cannabinoids Potency Method

Linked to recent vaping deaths, vitamin E acetate is emerging as a compound of concern for the cannabis industry. Here, we share a modified cannabinoids analysis that incorporates vitamin E acetate so that labs can screen for it within an existing potency test.

Click here for more information.

 

Fast 3-Minute, Standalone Vitamin E Acetate Analysis for Cannabis Labs

Adding vitamin E acetate to a potency analysis using the method above helps labs diversify their testing services within a single workflow. But, if speed is more important to you than simplification, we also developed this 3-minute analysis for vitamin E acetate alone.

Click here for more information.
 

Increase Profits with This Fast, Low-Solvent Use Cannabinoids Analysis

Suitable for high-throughput cannabis testing labs, this LC-UV method reliably separates 19 cannabinoids in a quick 10-minute analysis. Using only 3 mL of acetonitrile per run, labs can significantly decrease solvent use and the associated costs by adopting this procedure.

Click here for more information.

 

SFC-MS Separation of Cannabinoid Enantiomers

Regis’ chiral HPLC Whelk-O® 1 columns allow SFC-MS separation of cannabinoid enantiomers; including naturally occurring enantiomers (e.g. Cannabichromene (CBC) and those occurring from synthetic production (e.g. Δ9-THC).

Click here for more information.
 

Do you know the difference between Cannabis and THC isomers?

You know the need to separate Δ 8-TC and Δ 9-THC, but do you know what small difference between these two compounds results in such a large impact on potency?  This quick video pinpoints the minor change that has major effects.

Click here to watch the video.
 

Cannabinoid Profiling is a high-demand test that can be improved by increasing speed, expanding analyte lists, and reducing costs.

Click here for an application describing Fast, Low-Solvent Analysis of Cannabinoids Increases Lab Productivity and Decreases Solvent Costs.
 

High-Throughput Analysis of Cannabinoids by LC-UV

As the cannabis market grows, interest in more detailed analysis of cannabinoid profiles is expanding because more comprehensive data can be used for strain identification as well as to ensure more accurate potency testing.

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Pesticides

Analysis of 47 Pesticides in Cannabis for High-throughput analysis: Traditional dSPE vs. Positive Pressure dSPE in a 96-well plate

Cannabis markets are relatively new and vary significantly when it comes to the regulation of pesticides and mycotoxins, as well as uniform testing methods for potency.  Quality control methods are necessary to ensure product safety and appropriate cannabinoid profiling.  While several methods are being investigated to determine the best way to evaluate these compounds of interest, it is important to keep in mind that these methods need to be scalable and also able to be used for high throughput analyses.  This study examines using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction approach coupled with either traditional dSPE clean-up versus UCT’s dSPE clean-up in well-plate format and Hamilton’s MPE2 Positive Pressure Extraction/Evaporation Module for the analysis of 47 pesticides in marijuana. We demonstrate that for most compounds investigated, the high throughput clean-up method exhibits comparable results to traditional dSPE clean-up.

Click here for the full report.

 

Determination of 35 Pesticides and 3 Cannabinoids in Marijuana Edibles

Many cannabis producers and forensic toxicology labs are looking for fast, reliable and cost-effective methods to determine cannabis potency and pesticides in edibles.  All scientists agree that uniform testing policies and procedures need to be established as soon as possible and that overall sample clean-up is the main issue within these analyses.
 
Click here to read the full report.





The Analysis of Pesticides and Mycotoxins in Cannabis Brownies

Government regulations require the cannabis industry to test edible products for an extensive list of pesticides and mycotoxins. Here, an effective workflow for this complex analysis was developed in brownies, and optimization strategies are detailed in order to provide a starting point for similar matrices. Both LC-MS/MS and GC-MS/MS were employed and excellent results for LOQ, linearity, accuracy, and precision were attained for all the target compounds.

Click here to view the Analysis of Pesticides and Mycotoxins in Cannabis Brownies.


High-Quality Analysis of Pesticides in Cannabis (using QuEChERS, Cartridge SPE Cleanup, and GCxGC-TOFMS)

  • Quickly and effectively extract medical marijuana samples for pesticide analysis.
  • Cartridge SPE cleanup of dirty extracts improves GC inlet and column lifetimes.
  • Selective GC columns increase accuracy of pesticide determinations for complex samples.

Click here to view the High-Quality Analysis of Pesticides in Cannabis

 

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Mycotoxins

The Analysis of Mycotoxins and Pesticides in Cannabis Brownies

Government regulations require the cannabis industry to test edible products for an extensive list of pesticides and mycotoxins. Here, an effective workflow for this complex analysis was developed in brownies, and optimization strategies are detailed in order to provide a starting point for similar matrices. Both LC-MS/MS and GC-MS/MS were employed and excellent results for LOQ, linearity, accuracy, and precision were attained for all the target compounds.

Click here to view the Analysis of Pesticides and Mycotoxins in Cannabis Brownies.

 

High-Throughput Analysis of Mycotoxins in Cannabis CBD Oil Pairs Simplified Cleanup with LC-MS/MS Sensitivity

Aflatoxins and ochratoxins are an emerging concern in the cannabis industry as these secondary fungal metabolites can cause disease and death if consumed. Crops are susceptible to fungal growth from seed through storage, so there are many opportunities for contamination to occur. As a result, a growing need is emerging for testing to detect the presence of mycotoxins in both raw plant materials and finished cannabis products in order to protect consumer health and safety. The analysis of mycotoxins in cannabis oils is particularly challenging because the lipids in the sample can contribute isobaric matrix interferences in addition to general ion suppression that can reduce accuracy, particularly at low levels.

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The Analysis of Mycotoxins in CBD Oils by LC-MS/MS

Fungi that readily colonize crops such as cannabis are capable of producing secondary metabolites known as mycotoxins that can cause disease and death in humans.  The two primary types of mycotoxins associated with cannabis are aflatoxins and ochratoxins.

Crops are susceptible to contamination from seed through storage with most effort focused on mitigation during harvest and storage.  The thermal stability of mycotoxins presents a particular challenge as the likelihood is high that existing crop contamination will persist and concentrate during processing into oil and extracts causing levels to rise above allowable limits.
 
Click here to view the presentation first shown at ASMS 2019.

 

QuEChERS Method for the Determination of Mycotoxins

CrossTOX® is a low cost QuEChERS SPE Column for the Analysis of 16 Mycotoxins in one run!

  • Many labs analyze mycotoxins using a dilute-and-shoot method that is labour intensive and slow.
  • CrossTOX® improves these analyses by using a QuEChERS-based procedure that provides a cleaner extract than the syringe filter used in the dilute-and-shoot method (the syringe filter is no longer needed when using CrossTOX®)
  • Because the extract is cleaner, CrossTOX® allows a higher number of sample injections to be made before the LC-MS/MS needs to be cleaned or maintained.  That means your LC-MS/MS is running more and being serviced and cleaned less.
  • CrossTOX® saves significant cost per sample as much less internal standard is required.

Click here for additional information comparing CrossTOX® to dilute and shoot.

 

 

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Other

Thermal Analysis of CBD oil using a CDS Model 6150 Pyroprobe

With promise for treating a variety of ailments, cannabidiol (CBD oil) derived from the cannabis plant, has a growing interest in the pharmaceutical industry. The non-intoxicating extract is being credited with helping to treat many medical issues. Cut with hemp oil prior to use, it is a complex natural product, containing many volatile and non-volatile constituents. Successive thermal treatments using the Pyroprobe can clarify ingredients in natural materials such as CBD oil by separating ingredients based on their volatility, then pyrolyzing the non-volatile portion, like the oil itself.

 

Click here for more information.

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