Technical Information

 

The Chromatography and Sample Preparation Terminology Guide (PDF)
This terminology guide covers liquid chromatography (LC), Gas Chromatography (GC), and sample preparation.  (Link to ThermoFisher Scientific website)

 


GAS CHROMATOGRAPHY
 

Importance of Inert GC Pathways
When measuring trace-level impurities, your GC system needs to be optimized and inert. If it is not, low-level compounds cannot be adequately distinguished from the baseline and accurate identification and quantification become difficult or impossible. Sensitivity is strongly influenced by column factors including inertness and bleed.

Carrier Gases: Hydrogen as an Alternative to Helium
As the Helium shortage continues to grow, Helium prices are rocketing sky high and in some cases companies are being told they can not get any. Switching to Hydrogen as a carrier gas offers some great advantages.

The Impact of Using Guard Columns in Gas Chromatography (PDF)
Most of the time, impurities accumulate in the first meter(s) of the column. As the separation process in the capillary starts here, the unwanted interactions that take place will immediately impact the initial band width. Many users choose to connect guard columns in front of the analytical column to protect the analytical column from contamination.  These guard columns are deactivated and can be trimmed when polluted and eventually replaced.

 


LIQUID CHROMATOGRAPHY
 

Choosing the Right HPLC Column (PDF)
Follow our HPLC column selection flow chart as a starting point for your method development.  Click here to get started.

 


SAMPLE PREPARATION
 

Sample Preparation Handbook (PDF)
This handbook includes an overview of sample preparation techniques, industry-specific examples of challenging analyses, product information, and many cost saving tips and tricks.

Solid Phase Extraction (SPE)
SPE is a broad term to describe the digital (step wise) separation technique where liquids contact sorbents, and organic compounds or ions in the liquid adsorb to the functional group(s) of the sorbent.

QuEChERS
QuEChERS (pronounced Catchers), is an acronym for Quick, Easy, Cheap, Effective, Rugged and Safe. The process is a sample extraction and clean-up technique widely used for the analysis of multiple residues in food, forensic and environmental samples.

Soxhlet Extraction
Soxhlet Extraction is the most widely used method for the extraction of solids.  It is effective when your analytes have limited solubility and the sample impurity is insoluble in the desired solvent.

Derivatization
Derivatization reagents are designed to simplify chromatography by increasing sample volatility, allowing for simple GC-FID detection.